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Histological and immunohistochemical evaluations, reverse transcription-polymerase chain reaction (RT-PCR), and western blot analysis Histological and immunohistochemical staining were then employed to assess the cartilage regeneration ability in different groups, including H&E staining, toluidine blue staining, Safranin O/Fast Green staining, and immunohistochemical staining

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Always recalculate concentration when changing vial sizes

You're trying to hit a local target with a systemic flood that disappears almost immediately